Fig. 5.

Double labeling for SAP-102 (R, JH; 5 nm gold) and PSD-95 (M, TL; 15 nm gold) in the CA1 stratum radiatum of the hippocampus, at P2 (a, b), P10 (c, d), and P35 (e–j). Five nanometer gold in a, c, e, and g is indicated byarrowheads and shown at a higher magnification inb, d, f, and h, respectively.p, Presynaptic terminal. Postsynaptic structures include dendrite shafts (a, b), protuberances (c, d), and spines (e–j). Scale bar: a, c, 0.3 μm; e, g, i, j, 0.2 μm; b, d, f, h, 0.1 μm. Representative micrographs were chosen to illustrate the decrease in SAP-102 and the increase in PSD-95 in synapses with age, and to show examples of single- and double-labeled synapses in adults.