Table 2.

Strength^a and distribution of central CRF- or UCN-induced Fos expression relative to those of type 1 and type 2 CRF receptor mRNAs

	CRF-R1 mRNA	Fos-icv CRF	CRF-R2 mRNA	Fos-icv UCN
Forebrain
Isocortex
II–III	++	+++	−	+
IV	+++	++++	−	++
V	+	+	−	++
VI	++	+++	+	++
Claustrum	++	++	+	+
Olfactory regions
Main bulb
Periglomerular layer	++	++	−	+
Mitral layer	+++	++	−	+
Exterior plexiform layer	++	++	−	+
Granule cell layer	+++	+++	++	++
Anterior olfactory nucleus	++	++	+	+
Olfactory tubercle	++	+++	+	+
Piriform cortex	+++	+++	+	++
Hippocampal formation
Entorhinal area	++	++	++	++
Subiculum	++	+	+	++
Ammon's Horn	++	+	+	+/−
Dentate gyrus	++	+	+	+
Amygdala
Medial nucleus	++	++	++	++
Cortical nucleus (anterior/posterior)	++/+	++/+	++/+++	++/+
Central nucleus (lateral/medial)	−/+	+++/+	−/−	+++/+
Lateral nucleus	+	+	−	−
Basolateral nucleus	+++	++	−	+
Septum
Lateral nucleus	+	++	++++	++++
Medial nucleus/nucleus diagonal band	+++	+++	+	++
Bed nucleus stria terminalis	++	++	++	++
Anteromedial part	+	+	−	+
Anterolateral part	++	++	−	+
Oval nucleus	−	++	−	+++
Posterior part	++	++	+++	++
Basal ganglia
Caudoputamen	+++	++	−	+
Globus pallidus	++	+	+
Substantia innominata	++	+	−	−
Magnocellular preoptic nucleus	+++	++	+	+
Subthalamic nucleus	+++	+++	−	+
Substantia nigra
Pars compacta	+++	++	−	+
Pars reticulata	++	++	−	+
Ventral tegmental area	++	+	−	+
Thalamus
Anterior group	+	+	−	+
Midline group
Paraventricular nucleus	+	++	−	++
Nucleus Reuniens	++	++	−	++
Intralaminar group	+	++		++
Ventral group	++	++	−	+
Posterior complex	++	++	−	−
Reticular nucleus	−	−	−	−
Medial geniculate nucleus (medial/lateral)	++/+	++/++	−/−	+/−
Lateral geniculate nucleus (dorsal/ventral)	++/+++	++/+++	−/−	−/++
Hypothalamus
Periventricular zone
Suprachiasmatic nucleus	+	+	−	−
Paraventricular nucleus
Autonomic part	+	+	−	+
Parvicellular part	−	++	−	+++
Magnocellular part	−	−	+	++
Anterior periventricular nucleus	+	+	−	−
Arcuate nucleus	++	++	+	+
Posterior periventricular nucleus	+	+	+	+
Supraoptic nucleus	−	−	+	++
Medial zone
Medial preoptic nucleus	++	++	++	++
Dorsomedial nucleus	+++	+++	+	++
Ventromedial nucleus	−	+	+++	++
Premammillary nucleus	++	++	+	+
Supramammillary nucleus	++	+	−	−
Lateral zone
Lateral preoptic area	+	++	−	+
Lateral area	+	++	+	+
Posterior area	+++	+++	−	++
Brainstem
Sensory
Visual
Superior colliculus	++	+++	−	+
Olivary pretectal nucleus	++	+	−	+
Medial pretectal area	++	++	+	++
Somatosensory
Mesencephalic nucleus V	+++	+	+	+
Principal sensory nucleus V	+++	+++	−	+
Spinal nucleus V	+++	+++	−	+
External cuneate nucleus	++++	+++	−	++
Auditory
Cochlear nuclei	++	++	−	+
Lateral superior olive	++	+	−	−
Nucleus of the lateral lemniscus	+++	++	−	+
Inferior colliculus	+++	+++	−	++
Vestibular	++	++
Medial	++	++	−	+
Lateral	++	++	−	+
Spinal	+++	++	−	++
Visceral
Nucleus of the solitary tract	+	++	++	+++
Parabrachial nucleus (lateral medial)	+++/++	+++/+	−/−	+++/+
Motor
Oculomotor (III)	+	+	−	−
Facial nucleus (VII)	++	++	−	+
Nucleus ambiguous (X)	++	+	−	+
Reticular core
Periaqueductal gray	+	++	−	++
Ventral tegmental nucleus	++	++	−	++
Nucleus of the incertus	++++	++++	−	++++
Laterodorsal tegmental nucleus	++++	+++	−	++
Locus coeruleus	−	+++	−	++++
Raphé
Dorsal raphé	+	+++	+++	+++
Median raphé	++	+	++	++
Nucleus of the raphémagnus	++	++	−	+
Nucleus of the raphépallidus	+	++	−	++
Nucleus of the raphéobscurus	++	+	−	+
Interpedunuclar nucleus	++	++	++	++
Reticular formation
Pedunculopontine nucleus	++++	++	−	++
Gigantocellular reticular field	++	+	−	+++
Ventrolateral medulla (A1/C1)	−/+	++/++	−/−	++/++
Pre- and post-cerebellar
Pontine gray	++++	+++	−	++
Lateral reticular nucleus	++++	+++	−	++
Red nucleus	+++	++	−	−
Tegmental reticular nucleus	++	++	−	++
Inferior olive	++	++	−	+
Cerebellum
Deep nuclei	+++	++	−	+
Cortex
Purkinje layer	++	+	−	+
Molecular layer	++	++	−	+
Granule layer	+++	+++	−	++

^F2-aThe relative strength of the expression of each marker was rated by two independent observers without knowledge of the treatment status of the animals. Ratings reflect primarily the density of positively labeled cells, with (−) representing a complete lack of above-control levels of staining, (+) isolated positively labeled cells, and (++++) labeling in a substantial majority of all cells in a given cell group or field. Ratings separated by a slash (/) indicate those applicable to subregions of the particular cell group, as indicated at the left. Ratings of mRNA expression were adjusted secondarily on the basis of the strength of hybridization signal, but never by more than a single rating point. Ratings were based on analysis of complete series of sections through the brains of five rats that received icv injections of 1 μg CRF or UCN 2 hr before they were killed.