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Influence of the slope of concentration gradients on axonal length. The slope of gradients prepared from control membranes (open squares), or membranes containing Sema3A (filled circles) or Sema3C (filled triangles) did not influence the average axonal length both when axons that grow uphill (a) or downhill (b) the gradients were analyzed. The slope of the concentration gradients are shown as percent difference of membrane density over 25 μm, the average size of a cortical growth cone.
