Fig. 5.

Ca²⁺ inhibition of phospholipid vesiculation by dynamin I. A, Purified dynamin I and phosphatidylserine liposomes were incubated for 1 hr to allow dynamin assembly into helices around the liposomes. Addition of 1 mm GTP (bar), but not 1 mmGTP-γS, produced a decrease in light scattering corresponding to production of small vesicles from long tubule assemblies. Free Ca²⁺ was added 2 min before GTP addition.B, Inhibition of lipid vesiculation by Ca²⁺ is shown as a percentage of control [n = 4 (±SEM)].