Extended Data Fig. 3. cfDNA fragmentation in healthy individuals and patients with lung cancer.

a, cfDNA fragments lengths are shown for healthy individuals (n=30, gray) and patients with lung cancer (n=8, blue). b-d, cfDNA fragmentation profiles from healthy individuals (n=30) had high correlations while patients with lung cancer (n=8) had lower correlations to median fragmentation profiles of b, lymphocytes, c, lymphocyte nucleosome distances, and, d, healthy cfDNA. Pearson correlations are shown with box plots depicting minimum, 25^th percentile, median, 75^th percentile, and maximum values. e, High coverage (9x) whole-genome sequencing data were subsampled to 2x, 1x, 0.5x, 0.2x, and 0.1x fold coverage. Mean centered genome-wide fragmentation profiles in 5 Mb bins for 30 healthy individuals and 8 patients with lung cancer are depicted for each subsampled fold coverage with median profiles shown in blue. f, Pearson Correlation of subsampled profiles to initial profile at 9x coverage for healthy individuals and patients with lung cancer.