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. Author manuscript; available in PMC: 2020 Apr 4.
Published in final edited form as: Cell. 2019 Mar 14;177(2):256–271.e22. doi: 10.1016/j.cell.2019.02.014

Figure 3. Auditory GENUS reduces amyloid load in AC and HPC in 5XFAD mice.

Figure 3.

A. Relative soluble Aβ1–42 levels in AC and HPC in 6-month-old 5XFAD mice following 40 Hz, 8 Hz, 80 Hz, or random auditory stimulation for 1 hr/day for 7 days (1-week), normalized to non-stimulation control (n=19 mice no stim, n=19 mice 40 Hz, n=4 mice 8 Hz, n=7 80 Hz, n=6 random frequency, ****P<0.0001, KW-test with Dunn’s multiple comparison test).

B. As in A for insoluble Aβ1–42.

C. Immunohistochemistry with anti-Aβ (D54D2, green) antibody in 6-month-old AC of 5XFAD mice after auditory GENUS or no stimulation, for 1 week (n=7 mice/group, scale bar, 50 µm).

D. As in C for CA1.

E. Average number of Aβ-positive plaques in AC and CA1 (n=7 mice/group, *P<0.05, ****P<0.0001; unpaired Mann-Whitney Test).

F. Average area of Aβ-positive plaques in AC and CA1 (n=7 mice/group, **P<0.01, ***P<0.001; unpaired Mann-Whitney Test).

G. Immunohistochemistry with anti-Aβ (12F4, red) antibody in 6-month-old AC of 5XFAD mice after auditory GENUS or no stimulation, for 1 week (Inset, 20x, scale bar, 50 µm).

H. As in G for CA1.

I. Aβ (12F4) mean intensity value (12F4 antibody) normalized to non-stimulated controls (n=7 mice/ group, ****P<0.0001, unpaired Mann-Whitney Test). Circles indicate ‘n’, mean +/− s.e.m. in bar graphs unless otherwise noted, n.s. = not significant.