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. 2019 Sep 25;10:836. doi: 10.3389/fgene.2019.00836

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Copyright © 2019 Cappelli, Damiani, Mancini, Valzano, Rossi, Serrao, Ricci and Favia

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Plasmodium load in An. stephensi and An. gambiae mosquitoes challenged with Asaia. Evaluation of Plasmodium load has been performed at two time-points (three and five days after infected blood meal) in both An. stephensi (A) and An. gambiae (B). In the y axes the relative abundance of Plasmodium compared to the reference gene RpS7 is reported. Symbols in the plot represent individual mosquitoes (n = 9 per group of two experimental replicates, in total 18 mosquitoes) with horizontal lines indicating the medians (m). Statistical analysis was calculated with One-way ANOVA test and Dunn post-hoc test. **p < 0.01; ***p < 0.001.