Table 1.
Blacklip ablone genome assembly and annotation statistics.
| Parameter | Details | |||
|---|---|---|---|---|
| Genome Annotation | ||||
| Assembly Name: Method description | Total Length (bp) | Number of Scaffolds | N50 (bp) | N90 (bp) |
| Organism | Haliotis rubra (blacklip abalone) | |||
| Isolate | DU_JTF1 (inbred, brother/sister mating) | |||
| DU_PF1 (captive bred) | ||||
| Bioproject | PRJNA489521 | |||
| Biosample | SAMN09981888 (isolate DU_JTF1) | |||
| SAMN09981889 (isolate DU_PF1) | ||||
| GenBank assembly accession | GCA_003918875.1 (QKJH01) | |||
| Assembled Length | 1,378,265,264 bp | |||
| Scaffold N50 | 1,227,833 bp | |||
| Number of scaffolds | 2,854 | |||
| GC content | 40.52% | |||
| BUSCO completeness Metazoa odb9 (n = 978) |
91.6% Single-copy, 3% Duplicated, 1.6% Fragmented, 3.8% Missing | |||
| Number of predicted transcripts | 47,928 | |||
| Number of protein-coding genes | 44,137 | |||
| Number of proteins | ||||
| with InterProScan annotation | 24,743 (56%) 16,408 (37%) 6 (176 bp) 5 (2,001 bp) |
|||
| with Gene Ontology (GO) terms | ||||
| Average number (length) of exon per gene | ||||
| Average number (length) of intron per gene | ||||
| A1: Platanus (Illumina read assembly) | 1,441,198,393 | 419,307 | 15,704 | 1,602 |
| A2: wtdbg v1.2.8 (Nanopore read assembly) | 1,599,130,340 | 25,589 | 374,753 | 20,473 |
| A3: DBG2OLC (hybrid assembly) | 1,980,045,538 | 14,010 | 331,813 | 56,290 |
| A4:QuickMerge (meta-assemblies of A2 and A3) | 1,908,803,906 | 11,876 | 659,954 | 57,873 |
| A5: L_RNA_P (scaffolding of A4 with RNA-seq) | 1,908,833,106 | 11,584 | 705,847 | 59,076 |
| A6:PurgeHaplotigs (A5 haplotig removal) | 1,378,265,264 | 2,854 | 1,227,833 | 232,104 |