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. 2019 Sep 25;10:1033. doi: 10.3389/fpls.2019.01033

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Copyright © 2019 Nambeesan, Mattoo and Handa

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation and characterization of transgenic plants overexpressing yeast spermidine synthase (ySpdSyn). (A) The ySpdSyn construct was cloned under the CaMV35S promoter in pKYLX71 vector. Shown are levels of endogenous SlSpdSyn and the control SlACT using semiquantitative RT-PCR analysis indicating the expression of ySpdSyn and endogenous tomato SlSpdSyn in WT, C13, and C14 leaves. The tomato Actin gene was used as an internal control (SlACT). (B) Polyamine levels in WT, C13, and C14 leaves measured using HPLC. Shown are mean values and standard from three independent biological leaf replicates. Means followed by the same letter are not significantly different from each other based on one-way analysis of variance (α = 0.05) for a given polyamine (PA). Put, putrescine, Spd, spermidine; and Spm, spermine.