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. 2019 Sep 25;10:1033. doi: 10.3389/fpls.2019.01033

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Copyright © 2019 Nambeesan, Mattoo and Handa

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcript levels of SlDELLA and GA-biosynthesis genes during flower development of ySpdSyn and WT tomato lines. Flower development stages, early buds (0.1–0.7 cm) and late buds (0.8–1.5 cm), were harvested from plants grown in a green house. Expression of the tomato homologue of (A) DELLA (SlDELLA); (B) GA-20oxidase-1 (GA20ox1); (C) GA-20oxidase-2 (GA20ox2); and (D) GA-3oxidase-2 (GA3ox2), during flower development in wild type, C13 and C14 flower buds was analyzed using quantitative RT-PCR (Supplemental File 1). The relative gene expression analysis was performed by the ∆∆Ct method using tomato ACTIN as a reference gene. Data shown are mean + standard error based on ting three independent biological replicates of WT, C13, and C14 at early and late bud stage of flower development. Means followed by the same letter are not significantly different from each other based on one-way analysis of variance (α = 0.05) for a given tissue type of a gene.