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. 2019 Sep 25;10:1105. doi: 10.3389/fphar.2019.01105

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Copyright © 2019 Lee, Choi, Ha, Ji, Shin and Jung

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Change of mitochondrial reactive oxygen species (ROS) generation in hUtMC cells after treatment with GBH for 48 h. (A): MitoSOX Red, Mitotracker Green, and DAPI staining images obtained from hUtMC cells treated with GBH. (B): Mitochondrial superoxide (red) were detected by fluorescence 540/590 nm after staining the MitoROS 580 dye. (C): Apoptosis of hUtMCs treated with GBH in the presence/or absence of MitoTEMP, a mitochondria ROS scavenger, were analyzed using flow cytometry and the FlowJo software after staining with propidium iodide (PI) and annexin V-FITC. * P < 0.05, ** P < 0.01, *** P < 0.001, vs control. GBH, Gyejibongnyeong-hwan; UPA, ulipristal acetate; hUtMCs, human uterine myoma cells; MitoROS, mitochondrial superoxide.