Extended Data Table 1.
Cryo-EM data collection, refinement and validation statistics
| NLRP3-NEK7 Talos Arctica dataset | NLRP3-NEK7 Titan Krios dataset | |
|---|---|---|
| Data collection and processing | ||
| Magnification | 105,000 | 165,000 |
| Voltage (keV) | 200 | 300 |
| Electron exposure (e−/Å2) | ~59 | ~55 |
| Defocus range (μm) | −0.5 to −3.0 | −0.8 to −3.0 |
| Super Resolution pixel size (Å) | 0.589 | 0.42 |
| Symmetry imposed | C1 | C1 |
| Initial particle images (no.) | 1,086,055 | 1,850,332 |
| Final particle images (no.) | 98,340 | 108,771 |
| Map resolution (Å) | 4.3 | 3.8 |
| FSC threshold | 0.143 | 0.143 |
| Map resolution range (Å) | 3.5–5.8 | 2.5–6.6 |
| Refinement | ||
| Initial model used | ||
| Model resolution (Å) | 4.3 | |
| FSC threshold | 0.5 | |
| Model resolution range (Å) | 2.5–6.6 | |
| Map sharpening B factor (Å2) | −100 | |
| Model composition | ||
| Non-hydrogen atoms | 7979 | |
| Protein residues | 980 | |
| Ligands | 1 | |
| B factors (Å2) | ||
| Protein | 132.7 | |
| Ligand | 96.7 | |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.006 | |
| Bond angles (°) | 1.345 | |
| Validation | ||
| MolProbity score | 2.35 | |
| Clashscore | 8.92 | |
| Poor rotamers (%) | 1.91 | |
| Ramachandran plot | ||
| Favoured (%) | 84.96 | |
| Allowed (%) | 14.02 | |
| Disallowed (%) | 1.02 |