TABLE 2.
Stereological determination of Egr-1–positive cell density on the left and right hemispheres at the infragranular layers of the somatosensorial cortex for 2 and 6 h of recording of the induction of cortical spreading depression (CSD) in adult rats.
Group | Animal | Egr-1 cell | SCE | Thickness | Egr-1 cell | SCE | Thickness |
density RH | RH | RH | density LH | LH | LH | ||
2 h recording High number of CSD episodes | 4 | 77,075.12 | 0.04 | 28.30 | 163,850.09 | 0.03 | 29.16 |
6 | 66,525.43 | 0.04 | 29.57 | 104,606.94 | 0.03 | 30.16 | |
14 | 67,778.58 | 0.04 | 27.92 | 147,191.29 | 0.03 | 28.99 | |
24 | 122,969.38 | 0.03 | 28.61 | 110,417.15 | 0.03 | 29.72 | |
13 | 63,254.55 | 0.06 | 27.05 | 98,960.69 | 0.04 | 28.41 | |
15 | 75,121.43 | 0.05 | 24.15 | 109,653.23 | 0.04 | 26.97 | |
Mean | 78,787.53 | 0.04 | 27.60 | 122,446.563 | 0.03 | 28.90 | |
SD | 22,276.52 | 0.009 | 1.87 | 26,475.53 | 0.006 | 1.12 | |
2 h recording Low number of CSD episodes | 3 | 79,707.50 | 0.06 | 26.25 | 128,828.49 | 0.05 | 34.47 |
11 | 63,274.85 | 0.05 | 25.06 | 105,694.41 | 0.05 | 25.45 | |
29 | 65,865.10 | 0.05 | 29.92 | 81,687.37 | 0.04 | 31.08 | |
31 | 78,962.42 | 0.04 | 31.23 | 97,698.16 | 0.04 | 29.79 | |
34 | 56,375.91 | 0.05 | 29.87 | 87,055.36 | 0.04 | 29.87 | |
Mean | 68,837.16 | 0.05 | 28.47 | 100,192.76 | 0.04 | 30.13 | |
SD | 10,194.86 | 0.005 | 2.66 | 18,517.14 | 0.006 | 3.23 | |
6 h recording High number of CSD episodes | 18 | 119,353.3238 | 0.04 | 31.08 | 79,166.98099 | 0.06 | 31.34 |
20 | 159,225.4106 | 0.04 | 29.97 | 146,291.4382 | 0.04 | 31.08 | |
26 | 87,300.06771 | 0.05 | 29.58 | 103,398.8471 | 0.04 | 30.30 | |
27 | 118,105.1301 | 0.03 | 28.67 | 128,295.7078 | 0.03 | 28.14 | |
28 | 127,835.8445 | 0.04 | 30.51 | 119,502.0794 | 0.04 | 29.42 | |
Mean | 122,363.9553 | 0.04 | 29.96 | 115,331.0107 | 0.04 | 30.05 | |
SD | 25,716.66 | 0.006 | 0.92 | 79,166.98099 | 0.010 | 1.31 | |
6 h recording Low number of CSD episodes | 29 rep | 79,578.4467 | 0.03 | 30.55 | 77,361.2809 | 0.04 | 29.78 |
30 | 78,495.4937 | 0.04 | 30.44 | 82,902.15573 | 0.04 | 29.22 | |
32 | 78,738.9635 | 0.04 | 29.75 | 92,226.46693 | 0.04 | 30.37 | |
33 | 55,357.8865 | 0.05 | 29.09 | 69,011.61588 | 0.04 | 30.58 | |
39 | 73,257.0813 | 0.04 | 28.65 | 59,969.65573 | 0.05 | 28.03 | |
Mean | 73,085.5743 | 0.04 | 29.69 | 76,294.24 | 0.04 | 29.60 | |
SD | 10,218.68 | 0.007 | 0.83 | 12,431.4887 | 0.004 | 1.023 | |
2 h recording Sham | 9 | 72,696.24085 | 0.04 | 29.67 | 72,243.4081 | 0.04 | 27.29 |
10 | 72,077.6669 | 0.04 | 26.75 | 66,980.1333 | 0.04 | 28.15 | |
19 | 69,493.47595 | 0.04 | 30.02 | 53,485.9831 | 0.05 | 29.17 | |
Mean | 71,422.46124 | 0.04 | 28.81 | 64,236.5081 | 0.04 | 28.20 | |
SD | 8,698.9398 | 0.0015 | 1.80 | 9,675.012 | 0.003 | 0.94 | |
6 h recording Sham | 36 | 59,427.30555 | 0.04 | 27.29 | 89,995.3672 | 28.49 | 0.04 |
37 | 57,573.96491 | 0.04 | 28.15 | 73,798.953 | 29.19 | 0.04 | |
38 | 46,997.21448 | 0.05 | 29.17 | 51,662.4134 | 29.55 | 0.04 | |
Mean | 54,666.16165 | 0.04 | 28.20 | 71,8189112 | 29.08 | 0.04 | |
SD | 6,705.84 | 0.003 | 0.94 | 19,243.0315 | 0.54 | 0.005 |
2 and 6 h; 2 and 6 h of recording; high and low number, high and low number of CSD episodes; sham, zero episodes; LH and RH, left and right hemispheres; SCE, Schaeffer coefficient error; SD, standard deviation.