Figure 4.

Systemic administration of an FGFR1 antagonist decreases alcohol, but not sucrose, consumption and preference. A, Experimental timeline scheme: alcohol experiment. Mice were trained to consume alcohol in the intermittent access to 20% alcohol in two-bottle choice paradigm for 5 weeks before injections. The FGFR1 antagonist PD173074 (5 or 15 mg/kg, i.p.) or vehicle was injected 1 h before the beginning of an alcohol-drinking session in a counterbalanced within-subjects design. Alcohol and water intake were measured after 24 h. B, Amount of alcohol (g/kg) consumed. C, Preference for alcohol, calculated as the ratio of the volume of alcohol solution intake/volume of total fluid intake. D, Water intake (ml/kg). n = 8 per group. *p < 0.05, **p < 0.01. E, Experimental timeline scheme: sucrose experiment. Mice were trained to consume sucrose (1%) in the intermittent access two-bottle choice paradigm for 3 weeks before PD173074 injections. PD173074 (15 mg/kg, i.p.) or vehicle was injected 1 h before the beginning of an alcohol-drinking session in a counterbalanced within-subjects design. F, Amount of sucrose (ml/kg) consumed. G, Preference for sucrose, calculated as the ratio of the volume of sucrose solution intake/volume of total fluid intake. Bar graphs represent mean + SEM adjusted for a within-subjects design (Cousineau, 2005). n = 18 per group.