Table 1.
List of primers used in this study
| Primer ID | Sequence | Primer type | Gene |
|---|---|---|---|
| qYD21 | GCCACTTCGCCGTTGATGATG | qPCR Forward | crh-1a |
| qYD20 | TCCTCCGGCTCCTTCTTCATC | qPCR Reverse | crh-1a |
| qYD179 | GTAGATGCTTCACCATTACAGTTT | qPCR Forward | crh-1b |
| qYD23 | GGCAAGACGAGTGGCTGATT | qPCR Reverse | crh-1b |
| qYD22 | ATGTCAGCGAAAGGTAACGGATC | qPCR Forward | crh-1c |
| qYD20 | TCCTCCGGCTCCTTCTTCATC | qPCR Reverse | crh-1c |
| qYD24 | GTACCCAACAGCAACACGG | qPCR Forward | crh-1d |
| qYD25 | CCGCCGTTTCTAGATCTTTGAG | qPCR Reverse | crh-1d |
| qYD19 | ATGATGTTCCTCAGGGCATTACAA | qPCR Forward | crh-1e |
| qYD20 | TCCTCCGGCTCCTTCTTCATC | qPCR Reverse | crh-1e |
| qYD26 | ATGGAGTCACTGGTTTTCAATGG | qPCR Forward | crh-1f |
| qYD27 | GTACGGATTGTTGTTGGGATGG | qPCR Reverse | crh-1f |
| qYD09 | TACTCTTTCACCACCACCGC | qPCR Forward | act-1 |
| qYD10 | ACGGTGATGACTTGTCCGTC | qPCR Reverse | act-1 |
| YD157 | TGGAAGGAGGAGGAGATGGAAA | Genotyping Forward (external) | crh-1(tz2) |
| YD158 | GCAGTACAGCTCTTTCAGCGTT | Genotyping Forward (internal) | crh-1(tz2) |
| YD159 | AATTCGGCACAACGGACTGG | Genotyping Reverse (external) | crh-1(tz2) |
| YD172 | ATTACCTGCAGGCTGCAGGTCGACTCTAGAG | Cloning Forward | phsp16.41 |
| YD173 | GGCAGCTAGCATTTTTTCTACCGGTACCAATAC | Cloning Reverse | phsp16.41 |
| YD165 | ATTAGCTAGCATGGCCACAATGGCGAGCAC | Cloning Forward | crh-1a |
| YD164 | ATTACCATGGTCACATTCCGTCCTTTTCCTTTC | Cloning Reverse | crh-1a |
| YD165 | ATTAGCTAGCATGGCCACAATGGCGAGCAC | Cloning Forward | crh-1b |
| YD164 | ATTACCATGGTCACATTCCGTCCTTTTCCTTTC | Cloning Reverse | crh-1b |
| YD166 | ATTAGCTAGCATGTCAGCGAAAGGTAACGGAT | Cloning Forward | crh-1c |
| YD164 | ATTACCATGGTCACATTCCGTCCTTTTCCTTTC | Cloning Reverse | crh-1c |
| YD167 | ATTAGCTAGCATGGCCACAATGGCGAGCAC | Cloning Forward | crh-1d |
| YD164 | ATTACCATGGTCACATTCCGTCCTTTTCCTTTC | Cloning Reverse | crh-1d |
| YD168 | ATTAGCTAGCATGATGTTCCTCAGGGCATTACA | Cloning Forward | crh-1e |
| YD164 | ATTACCATGGTCACATTCCGTCCTTTTCCTTTC | Cloning Reverse | crh-1e |
| YD169 | ATTAGCTAGCATGGAGTCACTGGTTTTCAATGG | Cloning Forward | crh-1f |
| YD164 | ATTACCATGGTCACATTCCGTCCTTTTCCTTTC | Cloning Reverse | crh-1f |
| YD180 | CATAATAACCGGTAAAATGTACCCATACGACGTTCCAG | Cloning Forward | 3xHA |
| YD181 | CATAGCTAGCAGCGTAATCTGGGACGTCA | Cloning Reverse | 3xHA |
| YD320 | ATCAGCATGCATCTTCAGATGGGAGCAGTGG | Cloning Forward | prab-3 |
| YD321 | GCATGGATCCAAACTTGTCATCTGAAAATAGGGCT | Cloning Reverse | prab-3 |
| YD260 | ATAGCCTGCAGGAAGTGGACACTGAGAGAGAGAG | Cloning Forward | pnmr-1 |
| YD261 | ATAGCCCGGGCTGTAACAAAACTAAAGTTTGTCGTGTTC | Cloning Reverse | pnmr-1 |
| AB139 | GCGTCGACAAGTGACACCACGCTCACA | Cloning Forward | prig-3 |
| AB140 | CCCCCCGGGAGCTGTGAAATTTTTAGGCAGT | Cloning Reverse | prig-3 |
| YD313 | ACGTGCATGCGTTAAATATGTGGCCACAATGACAATTAT | Cloning Forward | pgcy-13 |
| YD314 | ATCAGGATCCGTCCTGAAAAATTATTGAAAGTTTGTAATAAG | Cloning Reverse | pgcy-13 |
| YD253 | ATACCTGCAGGGAATTTAATAACTAATACATATTATTTGGCACACTC | Cloning Forward | pcrh-1e |
| YD254 | ATAGGATCCATTATTCAGAAATTGAATAGAGAATTAGAATTAGAAAC | Cloning Reverse | pcrh-1e |
| YD318 | CTGCAAGCTTCACTTTTCTACGTTTCCATAATAATTACATAG | Cloning Forward | pcrh-1a |
| YD319 | ATACGGATCCAGCCGTGAGATGTCCGC | Cloning Reverse | pcrh-1a |
| YD205 | AGTAAGCTTGTTTACGCTCGCGAGGAGAC | Cloning Forward | pstr-2 |
| YD206 | AGTCTGCAGTTTTATGGATCACGAGTATTCG | Cloning Reverse | pstr-2 |
| crYD35 | ATCAGGTCTCCTCTTCTTTTCTAATACTTACCCTGGTTTTAGAGCTAGAAATAGCAAG | Guide RNA | crh-1e deletion |
| crYD65 | ATCAGGTCTCCTCTTCAAAATGTCAGCGAAAGGTAAGTTTTAGAGCTAGAAATAGCAAG | Guide RNA | crh-1c deletion |
| crYD48 | ATCAGGTCTCCTCTTGAGCATCATCAACGGCGAAGGTTTTAGAGCTAGAAATAGCAAG | Guide RNA | crh-1a,b,d deletion |
| crYD106 | ATCAGGTCTCCTCTTTGGGCTCGAGCTGTAGCCGCGTTTTAGAGCTAGAAATAGCAAG | Guide RNA | crh1(K247R/K266R) |
| crYD01 | GTTTCTCGAGCCATGGTTACCGGTCTC | Guide RNA | Universal |
| crYD44 | ATACGCATGCACACCTAAAATTTTCAGATTGAGTCTGG | Forward | 5′ Homology arm crh-1e deletion |
| crYD45 | ATCAACTAGTTGCGCCCTGTTGAACTGGGA | Reverse | 5′ Homology arm crh-1e deletion |
| crYD46 | ATCAGGGCCCAGAAAAGATTAGTGTGTGTGTGTTTG | Forward | 3′ Homology arm crh-1e deletion |
| crYD47 | ATCACTTAAGCGAGTTGGTTTTATTACACAGCAGAG | Reverse | 3′ Homology arm crh-1e deletion |
| crYD59 | ATCAACATGTGCGAATCGCTGATTGGTTGCAATTC | Forward | 5′ Homology arm crh-1c deletion |
| crYD60 | ATCAACTAGTTTTATACTCGTTTTGGTGTGCAAAAAGGC | Reverse | 5′ Homology arm crh-1c deletion |
| crYD61 | ATCAGGGCCCAATGACACAAGAATGCGAAAACATCT | Forward | 3′ Homology arm crh-1c deletion |
| crYD62 | ATCACTTAAGTTCGGCCAATTTTGCGAATTTTAAG | Reverse | 3′ Homology arm crh-1c deletion |
| crYD50 | ATCAGCATGCTCTCTTTCACCGCCAATTTTTGTG | Forward | 5′ Homology arm crh- 1a,b,d deletion |
| crYD51 | ATCAACTAGTGAGATGTCCGCCACTTATTTTTGTTTA | Reverse | 5′ Homology arm crh- 1a,b,d deletion |
| crYD52 | ATCAGGGCCCAGTAGAGTTTTAGTGGAAAAATTTCGAG | Forward | 3′ Homology arm crh- 1a,b,d deletion |
| crYD53 | ATCACTTAAGCGATATGAGGGCCTCCTATTAAGTT | Reverse | 3′ Homology arm crh- 1a,b,d deletion |
| crYD74 | ACTCGCATGCGAAGTGCAATGAAGCCAATGTTGG | Forward | 5′ Homology arm crh- 1(K247R/K2 66R) |
| crYD90 | ACTAACTAGTCAAATTTCCGCTCCAAA | Reverse | 5′ Homology |
| AAATTACCTTACGTTTTCTGCGGCACTCTTTCGCTGCCTCTCGATT | arm crh- 1(K247R/K2 66R) | ||
| crYD91 | ACTCGGGCCCGAGGGAAAAAAATTATTTTTTGGCTGAAAAATTGA | Forward | 3′ Homology arm crh- 1(K247R/K2 66R) |
| crYD92 | ACGACTTAAGTGAGTGCTTTGTTCTGATTTTCCAGC | Reverse | 3′ Homology arm crh- 1(K247R/K2 66R) |