Figure 4.

Impact of pharmacological PI3Kδ inactivation on Aβ levels in primary neuronal cell cultures. A, B, Primary mouse cortical neurons were treated with CAL-101 (1 μm) for 7 d. At DIV8, secreted and intracellular Aβ1–38, Aβ1–40, and Aβ1–42 were measured in supernatants (A) and lysates (B), respectively. Results are expressed as picograms per milliliter. C, Primary mouse cortical neurons were treated with vehicle (DMSO) or CAL-101 (0.1, 0.3 and 1 μm) for 7 d. On DIV8, neurons were subjected to the MTT assay of cell viability. Data are displayed as mean ± SEM, n = 5–6 in A and B, n = 6 in C. One-way ANOVA followed by Bonferroni's post hoc test comparing all groups with the vehicle control group in A–C, **p < 0.01, ***p < 0.001.