Fig. 3.

Control of CCR7 expression by Ets-1. (A) MDA-MB-231 cells were transfected with a reporter plasmid containing wild-type human CCR7 promoter or an empty vector plasmid (pGL3). These cells were co-transfected with various concentrations of plasmid containing Ets-1 with the Pointed domain deleted (dp51). The promoter activity was examined by luciferase assay. (B) MDA-MB-231 cells were transfected with a reporter plasmid containing wild-type human CCR7 promoter and a NF-κB expression plasmid (p65). Additionally, these cells were transfected with various amounts of Ets-1 expression plasmids. The promoter activity was examined by luciferase assay. (C) MDA-MB-231 cells were transfected with a reporter plasmid containing the wild-type human CCR7 promoter and an Ets-1 expression plasmid. Additionally, cells were transfected with various amounts of CBP expression plasmids. The promoter activity was examined by luciferase assay.