Fig. 4.

Ets-1 modulates tumor metastasis. (A) MDA-MB-231 cells were co-transfected with Ets-1 and GFP. After transfection, cells were added to the upper chamber of a transwell plate. The lower chamber was filled with medium without CCL21 (white bar) or with CCL21 (black bar). After 48 hours, GFP+ cells in the lower chamber were quantified. (B) MDA-MB-231 cells were transfected with indicated siRNA. Cells were then treated with CCL21 and subjected to wound healing assay. Pictures were acquired at 40 hours. Migration area was calculated with MRI wound healing tool of Image J software and expressed as percentage change of Ets-1 siRNA vs. Non-target control (NT-SiRNA).