Table 6. Comparison of sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), likelihood ratio (LRs) and accuracy of different methods calculated with 95% confidence intervals (CIs) for MB, PB, and leprosy patients after MDT.
| Result for specimen | BI | Simple PCR | STNPCR | SYBRGreen | ||||
| (n = 43) | Positive | Negative | Positive | Negative | Positive | Negative | Positive | Negative |
| MB (after MDT) Positive | 7 | 7 | 3 | 11 | 6 | 8 | 7 | 7 |
| MB (after MDT) Negative | 0 | 29 | 0 | 29 | 0 | 29 | 0 | 29 |
| Sensitivity (95% CI) | 50(24.04–75.95) | 21.42(5.70–51.1) | 42.85(18.81–70.35) | 50.00(24.04–75.95) | ||||
| Specificity (95% CI) | 100(85.43–100) | 100(85.43–100) | 100(85.43–100) | 100(85.43–100) | ||||
| PPV (95% CI) | 100(56.09–100) | 100(30.99–100) | 100(51.68–100) | 100(56.09–100) | ||||
| NPV (95% CI) | 80.55(63.43–91.19) | 72.50(55.86–84.85) | 78.37(61.33–89.57) | 80.55(63.43–91.1) | ||||
| Positive Likelihood Ratio (LR+) | Infinity(NaN-Infinity) | Infinity(NaN-Infinity) | Infinity(NaN-Infinity) | Infinity(NaN-Infinity) | ||||
| Negative Likelihood Ratio (LR-) | 50.00(29.61–84.42) | 78.57(59.76–103.29) | 57.14(36.30–89.94) | 50.0(29.61–84.42) | ||||
| Accuracy % | 83.72 | 74.41 | 81.39 | 83.72 | ||||
| Result for specimen | BI | Simple PCR | STNPCR | SYBRGreen | ||||
| (n = 44) | Positive | Negative | Positive | Negative | Positive | Negative | Positive | Negative |
| PB (after MDT) Positive | 0 | 15 | 2 | 13 | 4 | 11 | 3 | 12 |
| PB (after MDT) Negative | 0 | 29 | 0 | 29 | 0 | 29 | 0 | 29 |
| Sensitivity (95% CI) | 0(0–25.34) | 13.33(2.34–41.61) | 26.66(8.91–55.16) | 20.00(5.31–48.62) | ||||
| Specificity (95% CI) | 100(85.43–100) | 100(85.43–100) | 100(85.43–100) | 100(85.43–100) | ||||
| PPV (95% CI) | NaN(NaN-NaN) | 100(19.78–100) | 100(39.57–100) | 100(30.99–100) | ||||
| NPV (95% CI) | 65.90(49.99–79.06) | 69.04(52.75–81.88) | 72.50(55.86–84.85) | 70.73(54.26–83.35) | ||||
| Positive Likelihood Ratio (LR+) | NaN(NaN-NaN) | Infinity(NaN-Infinity) | Infinity(NaN-Infinity) | Infinity(NaN-Infinity) | ||||
| Negative Likelihood Ratio (LR-) | 100(100–100) | 86.66(71.06–105.69) | 73.33(54.04–99.50) | 80.00(62.11–103.03) | ||||
| Accuracy % | 65.90 | 70.45 | 75.00 | 72.72 | ||||
| Result for specimen | BI | Simple PCR | STNPCR | SYBRGreen | ||||
| (n = 56) | Positive | Negative | Positive | Negative | Positive | Negative | Positive | Negative |
| Leprosy (after MDT) Positive | 7 | 22 | 5 | 24 | 10 | 19 | 10 | 19 |
| Leprosy (after MDT) Negative | 0 | 29 | 0 | 29 | 0 | 29 | 0 | 29 |
| Sensitivity (95% CI) | 24.13(11.01–43.92) | 17.24(6.52–36.48) | 34.48(18.59–54.33) | 34.48(18.59–54.33) | ||||
| Specificity (95% CI) | 100(85.43–100) | 100(85.43–100) | 100(85.43–100) | 100(85.43–100) | ||||
| PPV (95% CI) | 100(56.09–100) | 100(46.29–100) | 100(65.54–100) | 100(65.54–100) | ||||
| NPV (95% CI) | 50.00(36.73–63.26) | 54.71(40.55–68.19) | 60.41(45.29–73.88) | 60.41(45.29–73.88) | ||||
| Positive Likelihood Ratio (LR+) | 56.86(42.32–70.37) | Infinity(NaN-Infinity) | Infinity(NaN-Infinity) | Infinity(NaN-Infinity) | ||||
| Negative Likelihood Ratio (LR-) | 100(100–100) | 82.75(70.09–97.71) | 65.51(50.31–85.31) | 65.51(50.31–85.31) | ||||
| Accuracy % | 62.06 | 58.62 | 67.24 | 67.24 | ||||