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. Author manuscript; available in PMC: 2019 Oct 2.
Published in final edited form as: J Bone Miner Res. 2013 Dec;28(12):2476–2489. doi: 10.1002/jbmr.1992

Fig. 1.

Fig. 1

Nf1 gene dose regulates TGF-β1 production by osteoblasts. (A) Serum TGF-β1 levels were measured by ELISA in WT and Nf1flox/−;Col2.3Cre mice. n = 4 mice per group. ****p < 0.0001. (B) TGF-β1 mRNA expression in WT, Nf1+/−, Nf1−/− osteoblast progenitors was examined by quantitative PCR. n = 3 technical replicates. The experiment was repeated twice with similar results using independent cell lines. **p < 0.01, ****p < 0.0001. (C) Protein extracts were examined by ELISA to determine TGF-β1 protein expression. n = 3 technical replicates. The assay was repeated twice with similar results using independent cell lines. **p < 0.01, ****p < 0.0001. (D) Serum TGF-β1 levels were measured by ELISA in human NF1 patients and healthy controls. n = 7 samples per group. **p < 0.01.