Fig. 2. Lipid mobility changes in NL4.3 Gag-iGFP HIV-1–infected Jurkat T cells.

Median lipid diffusion coefficients (D_a) as determined by sSTED-FCS measurements of 20 sites each from two independent virus preparations and infections. Box-and-whisker plots (horizontal line, median; box, 25 to 75% percentiles or IQR; whiskers, 10 to 90% measurements) showing D_a values inside assembly sites (red), outside assembly sites (green), and in noninfected control cells (blue). Data were collected for (A) ATTO647N-PI(4,5)P2, (B) cholesterol (Chol-PEG-KK114), (C) SM (ATTO647N-SM), and (D) PE (ATTO647N-DPPE) analogs. Statistical significance was assessed by Wilcoxon rank sum test. ns, not significant.