Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (H. sapiens) | HEK293 (Human embryonic kidney293 cells) | American Type Culture Collection | ||
| Cell line (M. musculus) | PS1/PS2-/- MEF (mouse embryonic fibroblast cells) | B. De Strooper (Katholieke Universiteit Leuven, Belgium) | ||
| Strain (M. musculus) | Psen1+/+; Psen1-/-; Psen2-/- | J. Shen (Brigham and Women Hospital, USA) | MGI: 1202717 | PMID: 9160754 |
| Antibody | anti-EphA3 C19 (rabbit polyclonal) | Santa Cruz Biotechnology | Cat. #: sc-919 RRID: AB_2099221 |
WB (1:1000) |
| Antibody | anti-EphA3 H80 | Santa Cruz Biotechnology | Cat. #: sc-25456 RRID: AB_2099214 |
ICC (1:200) IHC (1:200) |
| Antibody | anti-EphA3 L18 (rabbit polyclonal) | Santa Cruz Biotechnology | Cat. #: sc-920 RRID: AB_2099218 |
WB (1:1000) |
| Antibody | anti-EphA3 (mouse monoclonal) | ThermoFisher Scientific | Cat. #: 5E11F2 RRID: AB_10104885 |
WB (1:1000) IP (5 μg) |
| Antibody | anti-CrkII | BD Biosciences | Cat. #: 610035 RRID: AB_397451 |
WB (1:5000) IP (5 μl) |
| Antibody | anti-Neurofilament; SMI312 (mouse monoclonal) | Covance | Cat. #: SMI-312R RRID: AB_2314906 |
ICC (1:1000) |
| Antibody | anti-tau; TNT-1 (mouse monoclonal) | Merck Millipore | Cat. #: MAB3420 RRID: AB_94855 |
IHC (1:400) |
| Antibody | anti-Nestin; rat-401 (mouse monoclonal) | Abcam | Cat. #: ab11306 RRID: AB_1640723 |
IHC (1:200) |
| Antibody | anti-NeuN (mouse monoclonal) | Merck Millipore | Cat. #: MAB377 RRID: AB_2298772 |
IHC (1:1000) |
| Antibody | anti-MAP2 (mouse monoclonal) | Sigma-Aldrich | Cat. #: M9942 RRID: AB_477256 |
ICC (1:300) IHC (1:800) |
| Antibody | anti-Doublecortin (rabbit polyclonal) | Abcam | Cat. #: ab18723 RRID: AB_732011 |
IHC (1:1000) |
| Antibody | PS1; APS11 (mouse monoclonal) | Abcam | Cat. #: ab15456 RRID: AB_301867 |
ICC (1:200) IHC (1:200) |
| Antibody | PS1 loop (rabbit polyclonal) | Merck Millipore | Cat. #: AB5308 RRID: AB_91785 |
WB (1:4000) |
| Antibody | APP C-terminal Saeko (rabbit polyclonal; serum) | M. Shoji (Gunma Univ School Medicine, Japan) | - | WB (1:7500) |
| Antibody | ß-actin [AC-15] (mouse monoclonal) | Sigma-Aldrich | Cat. #: A1978 RRID: AB_476692 |
WB (1:60000) |
| Antibody | anti-Flag [FG4R] (mouse monoclonal) | Abcam | Cat. #: ab127420 RRID: AB_11157374 |
IP (10 μl) |
| Antibody | anti-HA (mouse monoclonal) | Cell Signaling Technology | Cat. #: 2362 | WB (1:1000) |
| Antibody | AlexaFluor 594-phalloidin | ThermoFisher Scientific | Cat. #: A12381 RRID: AB_2315633 |
ICC (1:50) |
| Antibody | GFP (chicken polyclonal) | Abcam | Cat. #: 13970 RRID: AB_371416 |
ICC (1:1000) |
| Recombinant DNA reagent | EphA3-GFP | P. W. Janes (Monash University, Australia) | ||
| Recombinant DNA reagent | EphA3-HA | This study | ||
| Recombinant DNA reagent | EphA3-∆ICD | P. W. Janes (Monash University, Australia) | ||
| Recombinant DNA reagent | EphA3-∆LBD | P. W. Janes (Monash University, Australia) | ||
| Recombinant DNA reagent | EphA3-∆PDZ | P. W. Janes (Monash University, Australia) | ||
| Recombinant DNA reagent | EphA3-ICD | This study | ||
| Recombinant DNA reagent | EphA3-ICD-Flag | This study | ||
| Recombinant DNA reagent | EphA3-ICD-pCAGIG | This study | ||
| Recombinant DNA reagent | RhoA T19N | X. R. Bustelo (Centro Investigación del Cáncer, CSIC, Spain) | ||
| Recombinant DNA reagent | RhoA Q63L | P. Crespo (IBBTEC, Santander, Spain) | ||
| Recombinant DNA reagent | NMIIA WT | A. Bresnick (Albert Einstein College Medicine, NY, USA) | ||
| Recombinant DNA reagent | NMIIA S1943A | A. Bresnick (Albert Einstein College of Medicine, NY, USA) | ||
| Sequenced-based reagent | Epha3 ShRNA | This study | ||
| Sequenced-based reagent | RT-qPCR primers | This study | See Materials and methods | |
| Peptide, recombinant protein | ephrin-A5 Fc chimera | R and D Systems | Cat. #: 374-EA | |
| Commercial assay or kit | RhoA G-LISA Activation Assay Kit | Cytoskeleton Inc | Cat. #: BK124 | |
| Commercial assay or kit | Rnease Mini kit | Qiagen | Cat. #: 74106 | |
| Chemical compound, drug | DAPT | Sigma-Aldrich | Cat. #: D5942 | 5 μM |
| Chemical compound, drug | L685,458 | Tocris | Cat. #: 2627 | 5 μM |
| Chemical compound, drug | GM6001 | Enzo | Cat. #: BML-EI300-0001 | 25 μM |
| Chemical compound, drug | 1,10-PNT | Sigma | Cat. #: 131377 | 83 μM |
| Chemical compound, drug | MMP9/13 | Calbiochem | Cat. #: 444252 | 10 μM |
| Chemical compound, drug | Y27632; ROCK-1 inhibitor | Calbiochem | Cat. #: 688000 | 10 μM |
| Software, algorithm | GraphPad Prism | GraphPad Prism (https://www.graphpad.com/) | RRID:SCR_015807 | Version 6 |
| Software, algorithm | ImageJ | ImageJ (https://imagej.nih.gov/ij/) | RRID:SCR_003070 | |
| Software, algorithm | Imaris | Imaris (https://imaris.oxinst.com/) | RRID:SCR_007370 | Version 8 |