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. Author manuscript; available in PMC: 2020 Jun 15.
Published in final edited form as: Mol Cell Endocrinol. 2019 Mar 25;490:15–20. doi: 10.1016/j.mce.2019.03.009

Fig. 2. H19 overexpression increases progesterone production synergistically with cAMP in vitro.

Fig. 2.

H19 overexpression was performed in KGN cells using empty vector plasmid as control. Cells were transfected in the presence and absence of dbcAMP (1 mm). 48 h after transfection, P concentration was determined by ELISA and results normalized to total RNA. The progesterone yield after H19 overexpression in KGN cells (third bar from left) normalized to cell number was comparable to that observed after cAMP stimulation (second bar from left), and H19 overexpression plus cAMP yielded additive effects on progesterone production (fourth bar from left). Analyses were performed from 3 biological replicates per group, evaluated as technical duplicates. P values were calculated using two-way ANOVA and multiple comparisons were corrected by the Tukey method. n = 3, * = p < 0.05; ** = p < 0.01. a = vector +cAMP vs vector; b = H19 vs vector; c = H19 vs H19 + cAMP; d = vector +cAMP vs H19 + cAMP. n = 3; **p < 0.01.