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. Author manuscript; available in PMC: 2020 Jun 15.
Published in final edited form as: Mol Cell Endocrinol. 2019 Mar 25;490:15–20. doi: 10.1016/j.mce.2019.03.009

Fig. 4. cAMP-stimulated H19 and Star expression is decreased after H19 KD.

Fig. 4.

KK1 cells were transfected with mouse-specific siH19 or negative control siRNA (siCon) in the presence and absence of dbcAMP (1 mM). RNA was extracted 48 h after transfection and H19 and Star RNA levels determined by RT-qPCR. cAMP treatment significantly increased both H19 and Star (**p < 0.01). cAMP-stimulated Star expression is significantly blunted after H19KD (**p < 0.01). Results are representative of three independent transfection experiments, evaluated as technical duplicates. P values were calculated using two-way ANOVA and multiple comparisons were corrected by the Tukey method. n = 3, * = p < 0.05; ** = p < 0.01.