Table 1. Cryo-EM data collection, refinement and validation statistics.
| Motor domain (EMD-4917, PDB 6RLA) |
Tail (EMD-4918, PDB 6RLB) |
|
|---|---|---|
| Data collection and processing | ||
| Magnification | 105,000 | 105,000 |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e–/Å2) | 49.6a, 46.8b, 45.4c | 49.6a, 46.8b, 45.4c |
| Defocus range (μm) | –1 to –3.5 | –1 to –3.5 |
| Pixel size (Å) | 1.39 | 1.39 |
| Symmetry imposed | C2 | C1 |
| Initial particle images (no.) | 229,969 | 461,684 |
| Final particle images (no.) | 57,265 | 68,623 |
| Map resolution (Å) | 3.9 | 4.5 |
| FSC threshold | 0.143 | 0.143 |
| Map resolution range (Å) | 3.7–5.5 | 4.3–8.0 |
| Refinement | ||
| Initial model used (PDB code) | 4RH7 | 5OWO, 6F1T, 6F1Y, 6F1U, 6F1Z, 2PG1, 2XQQ & de novo |
| Model resolution (Å) | 3.8 | 4.5 |
| FSC threshold | 0.143 | 0.143 |
| Model resolution range (Å) | 3.7–5.5 | 4.3–8.0 |
| Map sharpening B factor (Å2) | –100 | –125 |
| Model composition | ||
| Nonhydrogen atoms | 43,034 | 20,408 |
| Protein residues | 5,500 | 4,122 |
| Ligands | ADP: 6, ATP: 2, Mg: 2 | 0 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.007 | 0.007 |
| Bond angles (°) | 1.141 | 0.951 |
| Validation | ||
| MolProbity score | 1.65 | backbone model |
| Clashscore | 3.81 | 0.41 |
| Poor rotamers (%) | 0.35 | backbone model |
| Ramachandran plot | ||
| Favored (%) | 92.07 | 95.91 |
| Allowed (%) | 7.93 | 4.04 |
| Disallowed (%) | 0 | 0.05 |
Dataset 1a, 2b, 3c