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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Cell Metab. 2019 Jul 25;30(4):689–705.e6. doi: 10.1016/j.cmet.2019.07.002

Fig. 2.

Fig. 2.

A novel protocol for isolating native LBs from LD mice. (A) LB purification scheme. (B) Polysaccharide was purified at different steps in the protocol via the Pflüger method and quantitated via glucose measurement following hydrolysis. Initial tissue weights and total polysaccharide at each step are shown. (C) Polysaccharide yields normalized to tissue weight. Triplicate samples were removed from each fraction and each measured in triplicate. Mean ±SD are shown. (D) Phosphate content of LBs from skeletal muscle and normal rabbit muscle glycogen. Mean ±SD of triplicate measurements are shown. (E) Normalized iodine spectra of purified LBs compared to commercial liver glycogen and amylopectin. Spectra shown are an average of 3 replicates. (F) Brain, (G) heart, and (H) skeletal muscle LBs stained with Lugol’s solution and visualized using a Zeiss Axioimager Z1. See also Figure S2.