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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Clin Cancer Res. 2019 Jun 19;25(19):5901–5912. doi: 10.1158/1078-0432.CCR-18-4089

Figure 4.

Figure 4.

Polycythemia in mice with Mk-restricted Jak2V617F expression is transplantable. Two million Jak2V617F/+; Pf4-Cre+ (CD45.2) and WT (CD45.1) bone marrow cells were transplanted into lethally irradiated female two-month old WT (CD45.1) mice at a ratio of 90:10 or 10:90. The Jak2V617F/+; Pf4-Cre+ donors were female six-month old mice. (A-E) Hematological indices including (A) hematocrit, (B) hemoglobin, (C) platelet, and (D) white blood cell counts in peripheral blood and (E) CD45.2 cell chimerism were measured at indicated times. Line graphs depict mean ± SEM. 90:10 vs 10:90, P < 0.01 by two-way ANOVA (A, B, C, E). (F) Recipients of 90:10 bone marrow cells showed increased spleen weights. (G) Flow cytometric analysis showed that recipients of 90:10 cells had increased number erythroid populations in the spleen. H&E staining of spleen sections from recipients of 90:10 bone marrow revealed disruption of splenic architecture (H). Scale bars depict 100 microns. Bar graphs depict mean ± SEM. **P < 0.01 by the unpaired two-sided Student’s t test. n=4 animals per group. Data are representative of 2 independent experiments.