Figure 5:

Negative stain EM images of actin filaments show states of F-actin networks in the presence of Vt, MVt and MVt deletion mutant. Images are acquired at the same magnification (scale bar = 500 nm). Crosslinking or bundling of actin filaments (A) by Vt generates thick F-actin fibers (B). In contrast, MVt does not promote actin filament bundling (C), rather significantly reduces Vt-induced actin bundling (D). Removal of MVt H1 and N-terminal strap (ΔH1&NtS) promotes F-actin bundling similar to Vt (E), and in the presence of Vt shows similar F-actin bundling to Vt alone (F).