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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Structure. 2019 Aug 15;27(10):1527–1536.e3. doi: 10.1016/j.str.2019.07.013

Figure 7:

Figure 7:

F-actin assemblies mediated by Vt and MVt. (A) Vt promotes formation of linear F-actin bundles (B) WT MVt inhibits F-acting bundling and crosslinking. H1 and MVt insert form a protruding subdomain, which sterically occludes actin filaments from coming into interaction distance. (C) Removal of H1 from MVt, disrupts protruding structure and promotes F-actin bundling. (D) MVt cardiomyopathy mutations perturb formation of the insert-mediated ‘protruding’ structural element. Released H1 forms transient interactions with H2–5 four helix bundle of other Vt/MVt causing F-actin crosslinking. H2–5 is a four-helix bundle formed by helices 2-3-4-5 of MVt. H1 is the first helix of the five-helix bundle in Vt. H1’ is the first helix of the five-helix bundle of MVt.