KEY RESOURCE TABLE
| Reagent | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Phospho-Akt (Ser473) | Cell Signaling Technology | Cat# 9271 |
| Phospho-Akt (Thr308) | Cell Signaling Technology | Cat# 13038 |
| Akt | Cell Signaling Technology | Cat# 4685 |
| Phospho-mTOR (Ser2481) | Cell Signaling Technology | Cat# 2974 |
| mTOR (7C10) | Cell Signaling Technology | Cat# 2983 |
| Phospho-AS160 (Thr642) | Cell Signaling Technology | Cat# 8881 |
| Beta-tubulin | Cell Signaling Technology | Cat# 2146 |
| Actin | Millipore | Cat# MAB1501R |
| 12-LO Antibody (C5) | Santa Cruz Biotechnology | Cat# sc-365194 |
| CD45-PE-Cy7 | eBiosciences | Cat# 25-0451-82 |
| F4/80-APC-Cy7 | BioLegend | Cat# 123118 |
| CD206-Alex647 | BioRad | Cat# MCA2235A647 |
| CD11c-PE | BD Pharmingen | Cat# 561044 |
| Chemicals, peptides and recombinant protein and recombinant proteins | ||
| 12(S)-HEPE | Cayman Chemicals | Cat# 32550 |
| Insulin (adipocytes differentiation) | Sigma Aldrich | Cat# I6634 |
| Insulin (human, for glucose uptake) | Sigma Aldrich | Cat# I9278 |
| Insulin (Humulin, for ITT)) | Elly Lilli | N/A |
| NCTT956 | Sigma Aldrich | Cat# SML0499 |
| Norepinephrine | Sigma Aldrich | Cat# A0937 |
| Rosiglitazone | Sigma Aldrich | Cat# R2408 |
| Indomethacin | Sigma Aldrich | Cat# I7378 |
| Dexamethasone | Sigma Aldrich | Cat# D4902 |
| IBMX | Sigma Aldrich | Cat# I5879 |
| Rotenone | Sigma Aldrich | Cat# R8875 |
| Antimycin A | Sigma Aldrich | Cat# A8674 |
| FCCP | Sigma Aldrich | Cat# C2920 |
| Oligomycin | Millipore | Cat# 495455 |
| ZnSO4 | Sigma Aldrich | Cat# Z2876 |
| Ba(OH)2 | Sigma Aldrich | Cat# B4059 |
| Perchloric acid | Sigma Aldrich | Cat# 244252 |
| Pertussis toxin | Sigma Aldrich | Cat# P7208 |
| Bacalein | Tocris | Cat# 1761 |
| Melittin | Tocris | Cat# 1193 |
| CL316,243 | Tocris | Cat# 1499 |
| YM254890 | FujiFilm Wako Chemicals | Cat# 257-00631 |
| LOXBlock-1 | Donated by Epigen Biosciences | N/A |
| [3H]2-deoxyglucose | Perkin Elmer | Cat# 760562 |
| Critical Commercial Assays | ||
| Insulin Kit | Cristal Chemicals | Cat# 90080 |
| Cell Line Nucleofector kit | Lonza | Cat# VVCA-1003 |
| Pierce BCA Protein Assay kit | Thermo Fisher | Cat# 23225 |
| RNA extraction kit | Zymo Research | Cat# R2052 |
| Sybr Green Master Mix | Thermo Fisher | Cat# A25778 |
| cDNA Reverse Transcription Kit | Thermo Fisher | Cat# 4368813 |
| Oligonucleotides | ||
| Primers for qPCR | See Table S1 – this paper | N/A |
| Primers for gRNAs | See Method Details – this paper | N/A |
| Gnas siRNA | Dharmacon | Cat# R040646-00-0005 |
| Control siRNA | Dharmacon | Cat# D-001810-10-05 |
| Recombinant DNA | ||
| Alox 12 Myc-DDK-tagged | Origene | Cat# MR227302 |
| 1179_pAAV-U6-BbsI-gRNA-CB-EmGFP | Addgene | Cat# 89060 |
| pSpCas9(BB)-2A-Puro (PX459) V2.0 | Addgene | Cat# 62988 |
| Experimental Models: Cell Lines | ||
| WT-1 cells | Developed in Tseng lab | Tseng et al., 2004 |
| hWA A41 | Developed in Tseng lab | Xue et al., 2015; Kriszt et al., 2017 |
| hBA A41 | Developed in Tseng lab | Xue et al., 2015; Kriszt et al., 2017 |
| C2C12 | ATCC | Cat# CRL-1772 |
| 3T3-F442A | Sigma | Cat# 00070654 |
| Experimental Models: Organisms/Strains | ||
| C57BL6/J | Jackson Laboratories | Cat# 000664 |
| Atgl FLOX | Jackson Laboratories | Cat# 024278 |
| Cas9 knockin mice | Jackson Laboratories | Cat# 024857 |
| Myf5+ CRE | Jackson Laboratories | Cat# 007893 |
| STOCK Bmpr1atm2.1Bhr/Mmnc | MMRRC | Cat# 030469-UNC |
| Ucp1-CRE | Jackson Laboratories | Cat# 024670 |
| Adiponectin-CRE | Jackson Laboratories | Cat# 028020 |
| Datasets | ||
| Global Lipidomics Datasets | www.metabolomicsworkbench.org | DataTrack ID 1749, 1750 and 1751 |
| Algorithms and Software | ||
| GraphPad Prizm | GraphPad Software, San Diego, CA | Versions 7 and 8 |
| ImageJ | NIH | https://fiii.sc/ |