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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Mol Cancer Ther. 2019 Aug 8;18(10):1800–1810. doi: 10.1158/1535-7163.MCT-19-0046

Figure 3.

Figure 3.

Leelamine (LLM) treatment downregulated expression of fatty acid metabolism proteins in prostate cancer cells. A, Immunoblotting for ATP citrate lyase (ACLY), acetyl-CoA carboxylase 1 (ACC1), and fatty acid synthase (FASN) proteins using lysates from 22Rv1 and LNCaP cells after 12-hour or 24-hour treatment with ethanol or the indicated concentrations of LLM. The blots were probed with anti-β-Actin or anti-GAPDH antibody to correct for differences in protein loading. The numbers above bands represent quantitation of the protein level changes relative to corresponding ethanol-treated control. B, RT-PCR data showing effect of LLM treatment (12-hour or 24-hour treatment) on mRNA levels of ACLY, ACC1, and FASN. Results shown are mean ± SD (n=3). *Significantly different (P<0.05) compared with corresponding ethanol-treated control by one-way ANOVA followed by Dunnett’s test. Similar results were observed in replicate experiments.