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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Mol Cancer Ther. 2019 Aug 8;18(10):1800–1810. doi: 10.1158/1535-7163.MCT-19-0046

Figure 6.

Figure 6.

Sterol regulatory element binding protein 1 (SREBP1) overexpression partly attenuated leelamine (LLM)-mediated inhibition of accumulation of total free fatty acids and neutral lipids. A, Western blot for SREBP1 using lysates from empty vector transfected control cells (EV) and SREBP1 overexpressing cells (SREBP1). B, BODIPY staining in EV and SREBP1 (PC-3) cells after 24-hour treatment with ethanol or the indicated doses of LLM. C, Quantitation of number of neutral lipid droplets/cell. The results shown are mean ± SD (n=3). *Significantly different (P<0.05) compared with corresponding ethanol-treated control, and #between EV and SREBP1 cells by one-way ANOVA followed by Bonferroni’s test. D, Quantitation of intracellular levels of total free fatty acids in EV and SREBP1 cells (PC-3 and 22Rv1) after 24-hour treatment with ethanol or the indicated doses of LLM. Results shown are mean ± SD (n=2–3). Significantly different (P<0.05) *compared with corresponding ethanol-treated control, and #between EV and SREBP1 cells by one-way ANOVA followed by Bonferroni’s test. E, A mechanistic model showing LLM-mediated inhibition of fatty acid synthesis in prostate cancer cells.