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. 2019 Oct 2;9:14193. doi: 10.1038/s41598-019-50648-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ki-67 expression is diminished after AM-CM treatment. (A) HepG2 cells were seeded in 24-wells plate and treated with DMEM-F12 10% FBS (C), DMEM-F12 0% FBS (0% FBS), AM-CM 50% diluted (AM-CM 1/2), AM-CM 25% diluted (AM-CM 1/4), at indicated times. Cells were fixed and Ki-67 expression (green) was detected using Alexa-488 conjugated secondary antibody, at indicated times. Representative micrographs from HepG2 taken at 10X are shown. The nuclei were stained with DAPI (blue). (B,C) Graph shows the number of HepG2 nucleus positive for Ki-67 at 24 h (B) and 72 h (C) of treatment. Scale Bar: 100 μm. Results are expressed as means ± S.D. (n = 5) and one from a representative experiment is shown. ***p < 0.001 vs. 0% FBS; ^###p < 0.001 vs. 10% FBS.