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. 2019 Oct 2;9:14193. doi: 10.1038/s41598-019-50648-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pro and antitumoral miRs expression is modulated by AM-CM in HuH-7 cells. (A–D) HuH-7 cells were plated in complete DMEM-F12 medium supplemented with 10% FBS (C), in DMEM-F12 0% FBS (0% FBS), in AM-CM diluted at 50% (AM-CM 1/2), AM-CM at 25% (AM-CM 1/4) and incubated during 24 h (left) or 72 h (right) before RNA extraction. Total RNA was extracted as described in Materials and Methods. Relative expression of miR-15a (A), miR-210 (B), miR-206 (C), miR-145 (D) was determined by qRT-PCR. RNU6B was used as internal control for normalization. *p < 0.05, **p < 0.01, ***p < 0.001 vs. 0% FBS; ^#p < 0.05; ^##p < 0.01, ^###p < 0.001 vs 10% FBS.