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. 2019 Oct 2;9:14197. doi: 10.1038/s41598-019-50732-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reconstitution of miR-3613-3p suppresses CDC7 expression and reduces the number of colony formation in A375-R cells. (A) Endogenous expression of CDC7 was evaluated in presence and absence of 2.5 µM Vemurafenib after 24 and 48 h by Western blot analysis in parental (A375-P & WM983B-P) and resistant (A375-R & WM983B-R) cells. (B) A375-R was transfected with 50 nM miR-3613-3p mimic for 36 h, RNA was extracted and qPCR was performed. (C) Cell lysate was collected from the transfected cells with miR-3613-3p mimic (25 and 50 nM) and Western blot analysis was carried out using anti-CDC7 and anti-p-ERK1/2 antibodies. (D) Transfected and scrambled cells were cultured in 6-well plates for colony formation assay and kept for 10 days. After staining, colonies were counted and expressed as a percentage to control cells. *Depicts significance at p < 0.05.