Table 5.
Sequences of primer pairs used for amplification of the target and reference genes1.
| Gene2 | Strand | Sequence (5′ → 3′) | Ta | Product size (bp) | GenBank Accession No. |
|---|---|---|---|---|---|
| IL-2 | Forward | TTATGGAGCATCTCTATCATCAGCA | 63 | 122 | XM_01576098.1 |
| Reverse | CCTGGGTCTCAGTTGGTGTGTAG | ||||
| IL-6 | Forward | CTGTTCGCCTTTCAGACCTACC | 63 | 141 | NM_204628.1 |
| Reverse | GACCACTTCATCGGGATTTATCA | ||||
| MUC2 | Forward | ATGCGATGTTAACACAGGACTC | 60 | 110 | BX930545 |
| Reverse | GTGGAGCACAGCAGACTTTG | ||||
| CLDN1 | Forward | CATACTCCTGGGTCTGGTTGGT | 60 | 100 | NM_001013611.2 |
| Reverse | GACAGCCATCCGCATCTTCT | ||||
| OCLDN | Forward | CGCAGTCCAGCGGTTACTA | 58 | 178 | NM_205128.1 |
| Reverse | AGGATGACGATGAGGAACCCA | ||||
| GAPDH | Forward | TTGTCTCCTGTGACTTCAATGGTG | 63 | 128 | NM_204305 |
| Reverse | ACGGTTGCTGTATCCAAACTCAT | ||||
| β-Actin | Forward | CCTGGCACCTAGCACAATGAA | 63 | 175 | NM_205518.1 |
| Reverse | GGTTTAGAAGCATTTGCGGTG |
1For each gene the primer sequence for forward and reverse (5′ → 3′), the product size (bp), and the annealing temperature (Ta) in °C are shown.
2IL-, interleukin-; MUC2, mucin2; CLDN1, claudin1, OCLDN, occludin; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase.