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. 2019 Oct 2;9:14239. doi: 10.1038/s41598-019-50525-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genetic clusters identified with GENELAND (each color represents a genetic cluster) at Time 1 (T1; a) and Time 2 (T2; b), mean haplotype diversity (c) and nucleotide diversity + SD (d) including genetic differences and results of AMOVA analyses among sites, times (T1 and T2), and sites-times, mismatch distribution (e), and gene flow estimated with MIGRATE-n for E. analoga at T1 (f) and T2 (g). Arrows’ width represents the amount of immigrant numbers, circles’ size represents the value of Ne. NNI: northern non-impacted area, IA: impacted area and SNI: southern non-impacted area.