Table 2.
Results of FISH analysis with using of probes specific for the 1,137 kb deleted DNA fragment on metaphase chromosomes of fibroblasts derived from the founders carrying chromosomal rearrangements.
| Number of FO carrying rearrangement(s) | Number of examined metaphases | Number of metaphases with one labelled homolog of chromosome 6 (%)* | Number of metaphases with both labelled homologs of chromosome 6 (%)** | Absence of FISH signal on chromosome 6 (%) | Cells with trisomy of chromosome 6 (%)*** |
|---|---|---|---|---|---|
| Deletion | |||||
| 9 ♂ | 104 | 101 (97.1) | 3 (2.9) | 0 | |
| 11 ♂ | 87 | 85 (97.7) | 2 (2.3) | 0 | |
| 15 ♀ | 104 | 0 | 0 | 104 (100) | |
| 30 ♀ | 122 | 122 (100) | 0 | 0 | |
| 35 ♂ | 100 | 100 (100) | 0 | 0 | |
| Deletion and duplication | |||||
| 1 ♀ | 90 | 31 (34.4) | 57 (63.3) | 0 | 2 (2.3) |
| 20 ♀ | 118 | 69 (58.5) | 46 (39.0) | 0 | 3 (2.5) |
*FISH-positive signal (green) on one of the homologs of mouse chromosome 6 only with using of probes I15, J8 and E20 but both homologs contain red label with using by of the K19 probe;
**FISH-positive signals (green and red) on both homologs of mouse chromosome 6 with using of probes I15, J8, E20 and K19;
***Cells with trisomy of chromosome 6 was revealed in fibroblasts derived from the founders #1 and #20 only by K19 probe (red).