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. 2019 Oct 2;9:14185. doi: 10.1038/s41598-019-50726-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of SQSTM1/p62 in the retina of Ppt1 ko mice. (A) SQSTM1/p62-positive aggregates were detectable in the retina of 45-day-old mutants (Ab) and increased in number and size with increasing age of the animals (Ac,Ad). Note that the immunoreactive punctae were restricted to the inner nuclear layer at all ages analyzed (Ab–Ad). Wild-type retinas were devoid of SQSTM1/p62-positive punctae (for a 240-day-old animal, see Aa). (B) There was either no or only partial co-localization of SQSTM1/p62-positive aggregates (Ba) with LAMP1 (Bb) in P240 Ppt1 ko retinas (arrowheads in Ba–Bc). (C) Immunoblot analyses of retinas at P240 confirmed markedly elevated levels of SQSTM1/p62 in mutant retinas when compared to control retinas. GAPDH immunoblotting was performed as a loading control. Positions of the molecular mass markers are indicated. Uncropped blots are shown in Supplementary Fig. 5. gcl: ganglion cell layer; inl: inner nuclear layer; ipl: inner plexiform layer; onl: outer nuclear layer. Scale bar in (Ad) for (Aa–Ad): 100 µm; in (Bc) for (Ba–Bc): 10 µm.