Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 2;9:14184. doi: 10.1038/s41598-019-50738-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Detecting FS variants. (A) End-point RT-PCR analysis of the mSMC1A-1^{^}4 in mouse tumor cell lines and human hSMC1A-1^{^}4. (B) End-point RT-PCR analysis and RT-qPCR of the human hSMC1A-1^{^}4 expression in human primary breast tumor tissues and normal mammary tissues. All values are normalized relative to the expression levels in sample 1259 (set as 1). Data are mean 2^−ΔΔC of triplicates with SD. (C) Analysis of the human EST database for FS variants by exon skipping and trans-splicing. (D) Analysis of the frequency of the expression of the 35 trans-splicing variants in 50 human breast cancer cell lines and 54 primary human breast tumors. (E) Example of a sequence trace of the MS region in SEC62 dog and human genes in paired DNA/cDNA samples. (F) Summary sequencing results of microsatellite candidates in human (4 breast cancer cell lines) and dog (primary dog tumor tissues). (G) Ex vivo analysis of the MS INDEL in transcription and translation of the MS INDEL variants. eGFP was fused to the 3^rd reading frame after 11 A MS of SEC62 or after 11 non-MS nucleotides. The eGFP directly fused to 12 A MS was the positive control. The three different plasmids were transfected individually into 293 T cells and GFP fluorescence was measured 24 hrs after transfection. (H) FACS analysis of the GFP positive cells.