TABLE 1.

Primer sequences for the RT-PCR assay.

Gene symbol	Oligo	Primer sequence	Size (bp)
β-actin	Forward	5′- CCATCTATGAGGGTTACGC -3′	137
	Reverse	5′- GACAATTTCTCTTTCGGCT -3′
TNF-α	Forward	5′- GCTGGATCTTCAAAGTCGGGTGTA -3′	139
	Reverse	5′- TGTGAGTCTCAGCACACTTCCATC -3′
IL-1β	Forward	5′- CTCAGCCTGTGTGTTTGGGA -3′	209
	Reverse	5′- GGGACATTTGACGGACTCG -3′
IL-6	Forward	5′- ACGACATCAAACACAGCACC -3′	172
	Reverse	5′- TCGATCATCACGCTGGAGAA -3′
MyD88	Forward	5′- ACCATCGCCAGTGAGCTTAT -3′	206
	Reverse	5′- CAGATGGTCAGAAAGCGCAG -3′
ERK	Forward	5′- TCCAAGGGCTACACCAAGTC -3′	274
	Reverse	5′- TGCGATCCAATAAATCCAAA -3′
JNK	Forward	5′- ATTGCCTTTTGTCAGGGTTT -3′	140
	Reverse	5′- TACCGTTTGAGAACCGTGAA -3′
p38	Forward	5′- GTCGCAGAAAGAAAGACCCA -3′	130
	Reverse	5′- ATCAAACGCAGAGCAAACAG -3′
IκB-α	Forward	5′- GTTGGATTCGTTAAAAGAGGA -3′	137
	Reverse	5′- GGATAATGGCGAGATGTAGAT -3′
p65	Forward	5′- CGCAAGAGAACTGAAGGAA -3′	205
	Reverse	5′- AGAAAAAGGAGGTGGGTGG -3′
IL-10	Forward	5′- GGAGACCATTCTGCCAACA -3′	111
	Reverse	5′- CATTTCACCATATCCCGCT -3′
IFN-γ	Forward	5′- GTTTGCTGTTTTCGGGATGG -3′	138
	Reverse	5′- TTCGCAGGAAGATGGGGTGT -3′