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. 2019 Sep 26;9:959. doi: 10.3389/fonc.2019.00959

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Copyright © 2019 Dhahbi, Nunez Lopez, Schneider, Victoria, Saccon, Bharat, McClatchey, Atamna, Scierski, Golusinski, Golusinski and Masternak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confirming cell cycle-related functions for the cancer-associated Green coexpression module. (A) Enrichment for gene ontology (GO, biological processes) annotations among the Green module-overtargeted genes shown in Figure 4A. Companion table for the enrichment analysis is presented in Supplementary Table 4. (B–E) Validation of gene expression changes in tumor tissue (compared to adjacent normal/healthy tissue) by quantitative real time PCR (qPCR). Relative quantification was implemented using the 2^−ΔΔCt method for calculation of fold changes in gene expression. Expression of housekeeping gene β2-microglobulin was used as endogenous reference for normalization. Statistical analysis using the Student's t-test was implemented on the log transformed fold change data.