Skip to main content
. 2019 Aug 19;15(10):2240–2255. doi: 10.7150/ijbs.35356

Figure 6.

Figure 6

miR-27a acts through MMP2 to exert bystander effects. (A) Potential miR-27a binding site in the 5'-UTR of MMP2 and the effects of the binding site on luciferase activity. (B) The representative western blot image and the quantification of the levels of MMP2 expression in WS1 cells transfected with miR-27a mimics and NC. (C) The representative western blot image and the quantification of the levels of MMP2 expression in bystander WS1 cells after co-culture with unirradiated and irradiated HaCaT cells for 6 and 12 h. (D) The representative western blot image and the quantification of the levels of MMP2 expression in bystander WS1-pCMV and WS1-pCMV-SOD2 cells after co-culture with unirradiated and irradiated HaCaT cells for 12 h. (E) The representative western blot image and the quantification of the levels of MMP2 expression in bystander WS1 cells after co-culture with unirradiated and irradiated HaCaT cells pre-transfected with miR-27a inhibitors and NC for 12 h. (F) The representative western blot image and the quantification of the levels of MMP2 expression in recipient WS1 cells after culture with the exosomes from unirradiated and irradiated HaCaT cells collected at different times post radiation for 12 h. (G) The MMP2 mRNA expression levels in WS1 cells after infection with lentiviral particles containing three MMP2-targeting shRNAs. (H) The quantification of the area of the wound scratches of the recipient MMP2-downregulated WS1 cells after culture with the 3 h exosomes from unirradiated and irradiated HaCaT cells. All the data represent the means ± SEM from three independent experiments (n=3). *P<0.05, **P<0.01 and ***P<0.001 compared with the relative control.