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. 2019 Aug 24;15(11):2330–2339. doi: 10.7150/ijbs.33881

Figure 2.

Figure 2

The siRNA-mediated knockdown of FZD2 inhibited the growth of CAL-27 cells in vitro. The expression of FZD2 in tongue cancer cell lines CAL-27 and TCA-8113 and normal human oral epithelial cell line HOEC was detected by Western blot (A). After siRNA transfection in CAL-27 cells, the expression of FZD2 was measured by real-time PCR (B) and Western blotting (C), the cell proliferation was detected by CCK8 assay (C, P < 0.01 by One-Way ANOVA followed by Tukey's multiple Comparison test from 96 h to 144 h) and colony-formation assay (D, P<0.005 by One-Way ANOVA followed by Tukey's multiple Comparison test, n=3).