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. 2019 Aug 24;15(11):2330–2339. doi: 10.7150/ijbs.33881

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Knockdown of FZD2 represses tumorigenicity in vivo. After siRNA transfection in CAL-27 cells, the cells were injected into the flanks of nude mice, the tumor-bearing animals was were sacrificed in experimental observation for 28 days and the tumors were removed for weighing (A, P<0.001 by One-Way ANOVA followed by Tukey's multiple Comparison test) and photographing (B). The expression of FZD2 (upper panel), cleaved Caspase-9 (middle panel) and Ki-67 (lower panel) in the xenograft tumors were detected by immunohistochemistry (C) (DAB, 200 x) and the different groups of positive cells were counted and compared(D, P<0.001 by One-Way ANOVA followed by Tukey's multiple Comparison test).