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. 2019 Sep 7;15(11):2497–2508. doi: 10.7150/ijbs.37481

Figure 5.

Figure 5

Down-regulation of Akt signaling is required for xanthohumol-mediated glycolysis suppression. A, HCT116 and HT29 cells were treated with PD98059 or wortmannin. Cell lysates were subjected to IB analysis. B, HCT116 and HT29 cells were transfected with siCtrl or siAkt, cell lysates were subjected to IB analysis. C, HCT116 cells were transfected with Myr-Akt1, cell lysates were subjected to IB analysis. D and E, glucose consumption (D) and lactate production (E) in Myr-Akt1 transfected HCT116 cells. F, HT29 cells were transfected with Myr-Akt1, cell lysates were subjected to IB analysis. G and H, glucose consumption (G) and lactate production (H) in Myr-Akt1 transfected HT29 cells. I, HCT116 and HT29 cells were transfected with Myr-Akt1 and treated with xanthohumol, cell lysates were subjected to IB analysis. J, HCT116 cells were transfected with Myr-Akt1 and treated with xanthohumol, cleaved-caspase 3 was analyzed by immunofluorescence. Data are shown as mean values ± S.D. *p<0.05, **p<0.01, ***p<0.001, a significant difference between groups as indicated.