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. 2019 Sep 7;15(11):2448–2460. doi: 10.7150/ijbs.37241

Figure 5.

Figure 5

Schematic diagram of cell co-culture.Tumor conditioned medium (TCM) was collected from TM40D cells grown in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin for 24h. (A) TM40D cells were plated into 0.4μm transwell inserts and allowed to adhere to their supports for 24h (i); 1 × 104 BMSCs from RiCKO or Rictorf/f mice were cultured in TCM in a 24‑well plate for 72h to change into CAFs (ii); The seeded inserts were placed over the CAFs and co-cultured for 48h (iii). (B) 1 × 104 BMSCs from RiCKO or Rictorf/f mice were cultured in TCM in a 24‑well plate for 72h to convert to CAFs (i); TM40D were plated into 8 μm transwell chambers for co-culture with CAFs for 8 or 24h (ii).