TABLE 1.

qPCR primers used in this study

Primer	Sequencea	Amplicon size (bp)	Efficiency
Dros_rt_1 (Drosophila actin gene [51])	F: 5′-GGAAACCACGCAAATTCTCAGT-3′	140	1.96
	R: 5′-CGACAACCAGAGCAGCAACTT-3′
Universal bacterial primer (52)	F: 5′-ACTCCTACGGGAGGCAGCAGT-3′	172	1.85
	R: 5′-ATTACCGCGGCTGCTGGC-3′
Acetobacter spp. (51)	F: 5′-TAGTGGCGGACGGGTGAGTA-3′	134	1.96
	R: 5′-AATCAAACGCAGGCTCCTCC-3′
Lactobacillus spp. (51)	F: 5′-AGGTAACGGCTCACCATGGC-3′	108	1.98
	R: 5′-ATTCCCTACTGCTGCCTCCC-3′
RpLP0 (29)	F: 5′-CCGAAAAGTCTGTGCTTTGTTCT-3′	83	1.85
	R: 5′-CGCTGCCTTGTTCTCCCTAA-3′
Duox (this study)	F: 5′-CATGCGCTCCTTCCACAATG-3′	146	1.82
	R: 5′-CACCAAGAAGAAACAGCCGC-3′
p38c (30)	F: 5′-TACCTATCGCGAGATCCGTCT-3′	225	1.84
	R: 5′-ATGTACTTCAGTCCCCGCAGT-3′
Mkp3 (20)	F: 5′-GTGACGCTCGCCTACTTGAT-3′	102	1.82
	R: 5′-GAAGTGGAAGTTGGGCGATA-3′
Cad99C (21)	F: 5′-TCTTCGTGAAGCCAGTGGAC-3′
	R: 5′-ACGATAGCGGGTTACCGTGC-3′	123	1.84

^aF, forward; R, reverse.