FIG 4.

Individual P. palmivora nuclei stretch and move independently of surrounding nuclear flow. (A to F, video 6) Time-lapse imaging of axenically grown P. palmivora LILI-td-NT hyphae containing nuclei transitioning from passive (P-type) to active (A-type) movements (A to D) or, conversely, from A type to P type (E and F). (A) Nucleus slows down and stretches sternward. (B) Nucleus shrinks and starts moving together with the surrounding nuclear flow. (C) Same as panel B, but nucleus enters a branch. (D) Nucleus stretches and moves opposite the surrounding nuclear flow. (E) Same as panel D but entering a branch. (F) Nucleus stretches and moves opposite the surrounding nuclear flow and then tumbles and initiates a similar movement in the opposite direction. Yellow arrows indicate mass nuclear flow. White arrowheads indicate nuclei. Frequencies are given based on a total of 70 nuclei. Bar, 10 μm.