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Differential scanning fluorimetry-based ligand screening of PsPto-PscA-LBD. Shown are the melting temperature (Tm) changes for each of the 95 compounds present in the Biolog PM3B compound array of nitrogen sources with respect to the Tm of the ligand-free protein. The dashed line indicates the threshold of 2°C for significant hits. Data are the means and standard deviations from two assays.
